Paris, 9 February 20012
The technique developed by the team makes it possible to detect living Gram-negative type bacteria, which include pathogens such as Escherichia coli, Salmonella typhimurium and Legionella pneumophila. To do so, the bacteria are placed in contact with KDO, a sugar that bacteria use to synthesize a specific polysaccharide of their cell membrane. However this sugar is modified beforehand through the introduction of an azide function (made up of three atoms of nitrogen). Deceived, the bacteria incorporate the artificial sugar in their membrane. Then, thanks to a fluorescent molecule that attaches exclusively to the azide group, it is possible to identify and count living Gram-negative bacteria, the only ones to have assimilated the modified KDO.
The importance of these results stems from the fact that there is no rapid technique that allows living bacteria of interest to be simultaneously detected and counted. Furthermore, current methods used to count living bacteria are not entirely satisfactory: those requiring to put bacteria in a culture medium are slow (up to several weeks), whereas rapid methods can give false negatives or positives. This new technique combines reliability with rapidity in the detection of living bacteria. Consequently, it could quickly become an indispensable tool in terms of microbiological quality control and public health.
Experiments conducted by the researchers on Gram-negative type bacteria validate this method. In the future, the use of a specific sugar of each bacterium of interest could allow the detection of a very wide range of living pathogenic bacteria.
© LCB (CNRS/Aix-Marseille Université) and ICMMO (CNRS/Université Paris-Sud)
An Escherichia coli bacterium labeled by the incorporation of a modified KDO and coupling with a fluorescent molecule.
Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core, A. Dumont, A. Malleron, M. Awwad, S. Dukan*, B. Vauzeilles*, Angew. Chem. Int. Ed., in press.
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